Evaluation of the Effects of DNase Treatment on Signal Specificity in RT-PCR and In Situ RT-PCR
نویسندگان
چکیده
منابع مشابه
Evaluation of the effects of DNase treatment on signal specificity in RT-PCR and in situ RT-PCR.
In this study, the effects of DNase treatment on the specificity of reverse transcription (RT)-PCR have been investigated on different samples, containing RNA, DNA, DNA/RNA and DNA/cDNA. This was to evaluate the possibilities of getting specific results in a direct in situ RT-PCR. All DNA targets in the samples were eliminated after 1 h of DNase treatment. However, some DNA fragments still rema...
متن کاملQuantitative PCR and RT-PCR in virology.
I n the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in vivo, and the study of virus-host relations...
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RT in situ PCR allows for the routine and rapid detection of low copy viral and human RNAs. Success with RT in situ PCR is best accomplished with formalin fixed, paraffin embedded material, which allows the study of archival material. The key variable for RT in situ PCR is protease digestion. The optimal digestion time, which is determined by testing a variety of protease digestion times, is de...
متن کاملon translation of politeness strategies in dialogues involving female characters in translations and retranslations of novels translated before and after the islamic revolution of iran and their effects on the image of women: a polysystem theory approach
abstract reception environment has considerable effects on accepting a translation. as the expectations of a target culture and its values and needs change throughout history, its criteria for accepting a translation or rejecting it will change accordingly (gentzler, 2001). the expectations of iran, as the reception environment in the present study, have changed after the islamic revolution. i...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98254st04